Analysis of the presynaptic signaling mechanisms underlying the inhibition of LTP in rat dentate gyrus by the tyrosine kinase inhibitor, genistein

No abstract

Neuronal Activity Induces Synaptic Delivery of hnRNP A2/B1 by a BDNF-Dependent Mechanism in Cultured Hippocampal Neurons

Dendritic protein synthesis plays a critical role in several forms of synaptic plasticity, including BDNF (brain-derived neurotrophic factor)-mediated long-term synaptic potentiation (LTP). Dendritic transcripts are typically transported in a repressed state as components of large ribonucleoprotein complexes, and then translated upon stimulation at, or in the vicinity, of activated synapses. Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) is a trans-acting factor involved in dendritic mRNA trafficking, but how the distribution of the protein in dendrites is regulated has not been characterized. Here we found that a fraction of hnRNP A2/B1 is present at the synapse under resting conditions in cultured hippocampal neurons. Accordingly, this ribonucleoprotein was detected in free mRNP, monosomal, and polyribosomal fractions obtained from synaptoneurosomes. Neuronal activity and BDNF treatment increased hnRNP A2/B1 protein levels in the cell body and dendritic compartments, and induced the delivery of this protein to synaptic sites. The activitydependent accumulation of hnRNP A2/B1 at the synapse required, at least in part, the activation of TrkB receptors, presumably by BDNF. This neurotrophin also upregulated the hnRNP A2/B1 mRNA in the soma but was without effect on the abundance of neuritic hnRNP A2/B1 transcripts. These results show that the distribution of hnRNP A2/B1 is regulated by BDNF and by neuronal activity, an effect that may have a role in BDNF-induced synaptic plasticity events

[Ca2+]i regulation by glutamate receptor agonists in cultured chick retina cells

AbstractThe effect of glutamate receptor agonists on the intracellular free calcium concentration ([Ca2+]i), measured with Indo-1, was studied in populations of cultured chick embryonic retina cells. The agonists of the ionotropic glutamate receptors,N-methyl-d-aspartate (NMDA), kainate, and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) increased the [Ca2+]i through a composite effect, comprising Ca2+ permeating the receptor-associated channels, and Ca2+ entering through voltage-gated Ca2+ channels. Furthermore, the [Ca2+i responses to NMDA and AMPA also involved Ca2+ release from intracellular stores, which could not be mobilized by stimulation of the metabotropic receptor

HIT: a Human-Inspired Trust model

This paper presents a new approach to assign trust levels in ad hoc networks. Our system is inspired by the human concept of trust. The trust level considers the recommendation of trustworthy neighbors and their own experience. For the recommendation computation, we take into account not only the trust level, but also its accuracy and the relationship maturity. We also propose the Recommendation Exchange Protocol (REP), which minimizes the number of exchanged messages. The results show the efficacy of the system and the influence of main parameters.8th IFIP/IEEE International conference on Mobile and Wireless CommunicationRed de Universidades con Carreras en Informática (RedUNCI

Event-driven field estimation for wireless sensor networks

This paper introduces and analyzes a field estimation scheme for wireless sensor networks. Our scheme imitates the response of living beings to the surrounding events. The sensors define their periphery of attention based on their own readings. Readings differing from the expected behavior are considered events of interest and trigger the data transmission to the sink. The presented scheme is evaluated with real-site-collected data and the tradeoff between the amount of data sent to the sink and the reconstruction error is analyzed. Results show that signifcant reduction in the data transmission and, as a consequence, in the energy consumption of the network is achievable while keeping low the average reconstruction error.8th IFIP/IEEE International conference on Mobile and Wireless CommunicationRed de Universidades con Carreras en Informática (RedUNCI

Event-driven field estimation for wireless sensor networks

This paper introduces and analyzes a field estimation scheme for wireless sensor networks. Our scheme imitates the response of living beings to the surrounding events. The sensors define their periphery of attention based on their own readings. Readings differing from the expected behavior are considered events of interest and trigger the data transmission to the sink. The presented scheme is evaluated with real-site-collected data and the tradeoff between the amount of data sent to the sink and the reconstruction error is analyzed. Results show that signifcant reduction in the data transmission and, as a consequence, in the energy consumption of the network is achievable while keeping low the average reconstruction error.8th IFIP/IEEE International conference on Mobile and Wireless CommunicationRed de Universidades con Carreras en Informática (RedUNCI

On-line Detection of Glutamate Release from Culture Chick Retinospheroids

AbstractA continuous fluorometric assay was adapted to measure the release of endogenous glutamate from cultured chick retinospheroids. The results obtained with this technique are compared with the release of [3H]d-aspartate from monolayer cultures of chick retina cells. It is shown that although excitatory amino acids may be released in a Ca2+-dependent manner, most of the neurotransmitter release from cultured retina cells occurs by reversal of the glutamate transporter. The presence of extracellular Ca2+ may actually inhibit glutamate release by the cells present in the retinospheroids, or the [3H]d-aspartate release by cells in monolayers, when veratridine is the depolarizing agent. Copyright © 1996 Elsevier Science Ltd

Alterations in GABAA-Receptor Trafficking and Synaptic Dysfunction in Brain Disorders

GABAA receptors (GABAAR) are the major players in fast inhibitory neurotransmission in the central nervous system (CNS). Regulation of GABAAR trafficking and the control of their surface expression play important roles in the modulation of the strength of synaptic inhibition. Different pieces of evidence show that alterations in the surface distribution of GABAAR and dysregulation of their turnover impair the activity of inhibitory synapses. A diminished efficacy of inhibitory neurotransmission affects the excitatory/inhibitory balance and is a common feature of various disorders of the CNS characterized by an increased excitability of neuronal networks. The synaptic pool of GABAAR is mainly controlled through regulation of internalization, recycling and lateral diffusion of the receptors. Under physiological condition these mechanisms are finely coordinated to define the strength of GABAergic synapses. In this review article, we focus on the alteration in GABAAR trafficking with an impact on the function of inhibitory synapses in various disorders of the CNS. In particular we discuss how similar molecular mechanisms affecting the synaptic distribution of GABAAR and consequently the excitatory/inhibitory balance may be associated with a wide diversity of pathologies of the CNS, from psychiatric disorders to acute alterations leading to neuronal death. A better understanding of the cellular and molecular mechanisms that contribute to the impairment of GABAergic neurotransmission in these disorders, in particular the alterations in GABAAR trafficking and surface distribution, may lead to the identification of new pharmacological targets and to the development of novel therapeutic strategies